Paris Cancer Institute: CARPEM has a strong activity in fertility preservation. This program involves 3 main units of APHP.Centre-Univeriste de Paris: Reproductive Medicine, Reproductive Biology – Centre for the Study and Conservation of Human Sperm (CECOS) and the urology units working together.
APHP.Centre Assisted Reproductive Technology center (ART) benefits from all the authorizations for practicing fertility preservation dispensed by the Health Local Agency. APHP.Centre ART center is the most important ART center dedicated to fertility preservation in the Great Paris area and at the national level. It has been ISO 9001 certified and accredited according to ISO 15189 since 2018. This program took in charges 2444 new cases of fertility preservation during the last 3 years (2016-2018). In 2017, sperm preservation activity of our team was nearly 10% of the national activity, 8.9% for oocyte vitrification, 25.1% for ovarian cortex and 38.2% for testicular tissue (in collaboration with the fertility preservation center of the Saint-Louis APHP-site for these 2 last activities). In 2018, 516 new patients benefited from sperm preservation, 178 from oocytes vitrification, 56 from testicular tissue preservation and 73 from ovarian tissue preservation. This activity is increasing every year (e.g. more than 2 fold increase for oocyte vitrification in the last two years). Patients come mainly from medical departments of APHP.Centre – Université de Paris or hospitals affiliated to Paris University as Saint-Louis hospital or Curie Institute. Some patients, essentially for children or young people and male, are also referred by other cancer centers located outside from APHP.Centre.
Almost 30 embryologists, surgeons, psychologists, midwifes and biologists are mainly dedicated to fertility preservation patients’ care.
Guidelines have been built thanks our multi-disciplinary team. They are applied and regularly discussed with the medical teams we are cooperating with.
All these activities are structured around 1 annual cancer multidisciplinary team meeting including members of the fertility preservation program. Indications for preservation, essentially in women with gynecological cancer, are discussed once a week in different meetings dedicated to ART or gynecology surgery.
A remarkable feature of is that we are able to provide a multidisciplinary network for fertility preservation, from patients counseling, medical and surgical act achievement, to patients care for using gametes or tissues (except testicular tissue) in an ART program if needed. We are collecting all the complementary strengths within a same area to ensure a medical care throughout the process (medical, surgical, cryobiology, embryology, nurse, psychology, social, …).
Physicians surgeon and biologists of this program are strongly involved in clinical and translational research. One of the major aims of our team is to create strong interactions between researchers and clinicians in order to translate basic.
Clinical and translational research involves 8 senior researchers. This project benefits from a joint effort gathering researchers and clinical teams from: CECOS (Dr V. Barraud-Lange), laboratory of P. Fouchet, part of the Institute of cellular and molecular radiobiology (IRCM-CEA), the Urology units from hEGP (Pr N. Thiounn) and the Fertility preservation unit of Saint-Louis APHP site (Pr C. Poirot).
This research project is led by V. Barraud-Lange. The main translational research fields are about Fertility preservation and restauration techniques.
Besides these projects, we aim i) to develop new technologies allowing the restauration of male fertility from immature testicular tissue and germinal stem cells ii) to find new freezing procedures to enhance freezing efficiency for testicular and ovarian tissues. iii) to study epigenetic features that could be modified following the cryopreservation process, and in particular Zac1 expression and its epigenetic control after oocyte vitrification in a mouse model.
Immature testicular tissue cryopreservation is the single fertility preservation option in young prepubertal boys for whom spermatogenesis has not yet started. This technique is still experimental. Indeed, if pups have been obtained in primate, no fertility restauration has been described in humans from immature testicular tissue. One of our project aims to develop testicular tissue graft and germinal stem cell (GSC) transplantation as strategies of fertility restauration from immature testicular tissue. Our topics are to (a) identity phenotypic makers allowing purifying populations highly enriched in human GSC, (b) to develop methods of culture in order to amplify human GSC in vitro, (c) to devise methods of transplantation of immature testicular tissue or of amplified GSC suspension in order to restore an endogenous spermatogenesis.
The techniques of cryopreservation of germinal tissue should also be improved in order to ensure a high survival rate of cells after tissue thawing. We collaborate with Pr A. Pelé (INSERM U698, Paris XIII University) who has developed a new polymer that could be used for human tissue cryopreservation, CryoOgel (patent registration ongoing). This new polymer is currently tested for its application on ovarian cortex freezing (co-direction of a PhD thesis, Pr C. Patrat).
Lastly, consequences of oocyte cryopreservation on early conceptus are also studied. We are particularly interested in studying transcriptome and its epigenetic control in mouse pre implantation embryos conceived after in vitro fertilization from vitrified oocytes (Dr C. Chalas, Pr C. Patrat, M2-student).
The Fertility Preservation Program integrates 8 interns and 10 fellows each year.
Medical and surgical teams are widely involved in the university teaching of fertility preservation in University of Paris and in several national teaching degrees/master’s degrees in the field of procreation and oncology, as the DU Procreation and Cancer organized by Pr C. Poirot.
The Medical and surgical teams are in charge of a national teaching program “Diplome Universitaire de Gynecologie de l’infertilité et AMP” with a specific session on fertility preservation as a part of a Continuing Medical Education (DPC) to provide continuing medical education for physicians.
This course addresses the needs of practicing scientists, gynecologists, reproductive endocrinologists, residents, and fellows who wish to review and update their knowledge in this fertility preservation.
Based on our skillness we would like to develop immature testicular tissue graft and germinal stem cells transplantation:
|Name Surname||Title/Position||Speciality||Research Unit||Resarch Team|
|Virginie BARRAUD-LANGE||MCU-PH||Biologist||INSERM U1016, Institut Cochin||From Gametes to Birth, Team Dr Vaiman|
|Bruno BORGHESE||PU-PH||Gynecologist||INSERM U1016, Institut Cochin||Development, Reproduction and Cancer, Team Dr Vaiman|
|Mathilde BOURDON||CCA||Gynecologist||INSERM U1016, Institut Cochin||Development, Reproduction and Cancer, Team Pr Batteux|
|Céline CHALAS||PH||Biologist||INSERM U1016, Institut Cochin||From Gametes to Birth, Team Dr Vaiman|
|Charles CHAPRON||PU-PH||Gynecologist||INSERM U1016, Institut Cochin||Development, Reproduction and Cancer, Team Pr Batteux|
|Véronique DROUINEAUD||PH, Full time physician||Biologist|
|Louis MARCELLIN||MCU-PH||Gynecologist||INSERM U1016, Institut Cochin||Development, Reproduction and Cancer, Team Pr Batteux|
|Charlotte PANISSARD||M2-Student||Biologist||INSERM U1016, Institut Cochin||From Gametes to Birth, Team Dr Vaiman|
|Catherine PATRAT||PU-PH||Biologist||INSERM U1016, Institut Cochin||From Gametes to Birth, Team Dr Vaiman|
|Khaled POCATE-CHERIET||PH||Biologist||INSERM U1016, Institut Cochin||Development, Reproduction and Cancer, Team Pr Batteux|
|Pietro SANTULLI||PU-PH||Gynecologist||INSERM U1016, Institut Cochin||Development, Reproduction and Cancer, Team Pr Batteux|
The selected 10 following publications highlighted the strong collaboration between physicians and researchers:
|Number of events per year||2016||2017||2018|
|New sperm preservations||620||516||516|
|New oocyte preservations||85||149||178|
|New testicular tissue preservations||47||47||56|
|New ovarian cortex preservations||70||87||73|
Centre Universitaire des Saints-Pères Etage 4 – Pièce 446B 45 rue des Saints-Pères -75006 Paris
Carina Binet : Secrétaire Général du CARPEM
Tél. : 01 76 53 43 85 – firstname.lastname@example.org
Aurore Hattabi, PhD : Coordinatrice Scientifique du CARPEM
Tél : 01 76 53 43 85 – email@example.com